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KingFisher精彩回顾——深耕细作，步履不停，携手2024

来源：赛默飞世尔科技生命科学产品分类：成果 2024-01-26 09:25:54 13阅读次数

从1999年第一台KingFisher全自动磁珠纯化仪上市，到2021年全新升级型号KingFisher Apex的问世，KingFisher已和大家相伴20余载。一直以来，KingFisher在磁珠法提取纯化方向上深耕细作，推陈出新，志以最优质的性能服务客户，引领市场。

2023年作为疫情后“重启”的第一年，充满了挑战和机遇，KingFisher作为磁珠纯化领域的“解语花”，往期和大家分享了众多干货。让小编带大家来做KingFisher往期大盘点吧！

KingFisher专利技术——磁珠纯化金标准

1993年芬兰科学家发明磁棒法分离磁珠的KingFisher专利技术，作为最早把磁棒法专利转化为成熟产品的解决方案供应商，我们拥有20余年的样本纯化经验。KingFisher采用特制的磁棒，外包经特殊疏水处理的磁套，在不同液体孔位间吸取、转移和释放磁珠，无需吸液、冲洗，无气溶胶产生，无交叉污染，在提高产物质量、较高的一致性和可重复性的同时，极大地加快检测速度。

图1. KingFisher专利技术示意图

精彩回顾

磁珠富集病原体，污水检测“黑科技”

随着疫情的结束，城市污水监测成为了动态评估新型冠状病毒感染疫情流行强度、变化趋势及病毒变异情况的重要手段。

赛默飞作为生物行业领先者，率先开发出了依靠KingFisher和MagMAX Ultra污水核酸分离试剂盒的针对污水样本中病毒富集及提取的全流程解决方案。

图2. 10 mL 污水样本磁珠法浓缩自动化工作流程

动物疫病检测，赛默飞KingFisher助力复杂样本前处理

动物疫病检测是动物疫病防控中十分重要的一部分。赛默飞提供全流程动物疫病分子生物学检测方案，使用 KingFisher 全自动核酸提取仪进行病毒/细菌核酸提取，后续通过 Applied Biosystems? 实时荧光定量PCR仪进行检测，拥有准确、简单、快速的特点。

图4. 赛默飞动物疫病分子生物学检测流程

制备未来，KingFisher加速抗体药物研发进程

全球范围内抗体药物市场庞大且未来市场广阔，是生物制药中复合增长率最高的一类药物。随着抗体药物的深入研究与突破，其治疗领域将会进一步扩大。

KingFisher可以从各种类型样本中快速、高效、一致地进行核酸、蛋白、细胞的纯化，参与到抗体药物研发的整个流程中，加速抗体药研发进程。

图5. KingFisher在抗体药物研发中的应用

自动化整合双利器，助力 mRNA 疫苗快速研发

mRNA疫苗的研发流程通常涉及到许多需要高通量操作的实验，赛默飞实验室自动化方案可以帮助客户使实验流程自动化、标准化，加速mRNA疫苗的研发。

使用机械臂整合液体工作站，KingFisher Presto磁珠纯化仪，ATC自动化PCR仪，Vanquish UHPLC系统等仪器，可以自动化完成mRNA体外转录和纯化，mRNA加帽、加尾效率检测等流程，加速mRNA疫苗研发。

更多精彩内容

狗狗咬人啦，怎么分辨出是哪只小“坏蛋”？

遗传病三级预防，KingFisher助力基因检测

呼吸道传染病核酸检测及耐药性检测方案

新的一年，想要小编继续和大家分享哪些有关KingFisher的精彩内容呢？欢迎大家扫下方二维码参与投票，即有机会获得KingFisher Apex定制积木一套，赶紧扫码参与吧！

如果想了解更多产品信息，请点击“阅读原文”

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[2]Declaration of app\common\model\News::getAllList($filter = Array, $limit = 16, $order = Array, $with = Array) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\Company::getAllList($filter = Array, $limit = 20) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
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[8]Only variables should be passed by reference
[2]Declaration of app\common\model\NewsComment::getAllList($filter = Array, $limit = 16) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8192]Function create_function() is deprecated
[8192]Function create_function() is deprecated
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[2]Declaration of app\common\model\Topic::getList($filter = Array) should be compatible with app\common\model\BaseModel::getList($filter = Array, $sort = Array)
[2]Declaration of app\common\model\Topic::getAllList($filter = Array, $field = 'id,md5,tit...', $limit = 0) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\TopicInfoList::getAllList($filter = Array, $limit = 8) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
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[ SQL ] SELECT `id`,`name` FROM `news_category` WHERE `is_show` = 1 [ RunTime:0.000439s ]
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[ SQL ] SELECT * FROM `news_text` WHERE `news_id` = 4595 [ RunTime:0.039606s ]
[ SQL ] SHOW COLUMNS FROM `news_belong_category` [ RunTime:0.000949s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 4595 [ RunTime:0.002185s ]
[ SQL ] SELECT * FROM `news_category` WHERE `id` IN (5) [ RunTime:0.000218s ]
[ SQL ] SHOW COLUMNS FROM `user_follow` [ RunTime:0.000975s ]
[ SQL ] SELECT `id` FROM `user_follow` WHERE `user_id` IS NULL AND `follow_user_id` = 378 LIMIT 1 [ RunTime:0.000198s ]
[ SQL ] SHOW COLUMNS FROM `news_comment` [ RunTime:0.000756s ]
[ SQL ] SELECT * FROM `news_comment` WHERE `news_id` = 4595 AND `parent_id` = 0 ORDER BY `id` DESC [ RunTime:0.000370s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `news_comment` WHERE `news_id` = 4595 LIMIT 1 [ RunTime:0.000206s ]
[ SQL ] SELECT * FROM `news` WHERE `is_show` = 1 AND `review_status` = 1 AND `id` IN (4595,7908,7485,7409,7364,7104,6881,6766,6765,6344) ORDER BY field(id,4595,7908,7485,7409,7364,7104,6881,6766,6765,6344) [ RunTime:0.000436s ]
[ SQL ] SELECT * FROM `user_info` WHERE `user_id` IN (378,565,210,306,307,276) [ RunTime:0.000716s ]
[ SQL ] SHOW COLUMNS FROM `topic` [ RunTime:0.000886s ]
[ SQL ] SELECT `id`,`md5`,`title`,`created_time` FROM `topic` WHERE `review_status` = 1 ORDER BY `id` DESC LIMIT 4 [ RunTime:0.000169s ]
[ SQL ] SHOW COLUMNS FROM `topic_info_list` [ RunTime:0.000820s ]
[ SQL ] SELECT * FROM `topic_info_list` WHERE `topic_id` = 1 ORDER BY `id` DESC LIMIT 2 [ RunTime:0.000174s ]
[ SQL ] SELECT * FROM `news` WHERE `id` IN (864,849) [ RunTime:0.000181s ]
[ SQL ] SELECT `id`,`md5`,`title`,`created_time` FROM `news` WHERE `review_status` = 1 AND `is_show` = 1 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.000217s ]
[ SQL ] SHOW COLUMNS FROM `user` [ RunTime:0.000739s ]
[ SQL ] SELECT `a`.* FROM `user` `a` INNER JOIN `news` `b` ON `a`.`id`=`b`.`user_id` GROUP BY `a`.`id` ORDER BY `b`.`id` DESC LIMIT 10 [ RunTime:0.009773s ]
[ SQL ] SELECT * FROM `user_info` WHERE `user_id` IN (348,351,466,458,209,372,521,819,385,657) [ RunTime:0.000771s ]
[ SQL ] SHOW COLUMNS FROM `seo` [ RunTime:0.000803s ]
[ SQL ] SELECT * FROM `seo` WHERE `column_type` = '02' AND `page_type` = 'news' AND `info_id` = 4595 LIMIT 1 [ RunTime:0.000292s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 4595 [ RunTime:0.001754s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7908 [ RunTime:0.001700s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7485 [ RunTime:0.001698s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7409 [ RunTime:0.001738s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7364 [ RunTime:0.001705s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7104 [ RunTime:0.001664s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6881 [ RunTime:0.001666s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6766 [ RunTime:0.001650s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6765 [ RunTime:0.001640s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6344 [ RunTime:0.001683s ]
[ SQL ] SELECT * FROM `user` WHERE `id` = 378 LIMIT 1 [ RunTime:0.000207s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 348 LIMIT 1 [ RunTime:0.000216s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 348 LIMIT 1 [ RunTime:0.000149s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 351 LIMIT 1 [ RunTime:0.000164s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 351 LIMIT 1 [ RunTime:0.000138s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 466 LIMIT 1 [ RunTime:0.000147s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 466 LIMIT 1 [ RunTime:0.000174s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 458 LIMIT 1 [ RunTime:0.000155s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 458 LIMIT 1 [ RunTime:0.000155s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 209 LIMIT 1 [ RunTime:0.000135s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 209 LIMIT 1 [ RunTime:0.000131s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 372 LIMIT 1 [ RunTime:0.000134s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 372 LIMIT 1 [ RunTime:0.000133s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 521 LIMIT 1 [ RunTime:0.000129s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 521 LIMIT 1 [ RunTime:0.000146s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 819 LIMIT 1 [ RunTime:0.000137s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 819 LIMIT 1 [ RunTime:0.000140s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 385 LIMIT 1 [ RunTime:0.000132s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 385 LIMIT 1 [ RunTime:0.000129s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 657 LIMIT 1 [ RunTime:0.000142s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 657 LIMIT 1 [ RunTime:0.000203s ]

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