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Empower控制质谱系列使用小贴士（一） | 沃特世质谱以旧换新现在进行时

来源：沃特世科技（上海）有限公司(Waters) 分类：成果 2024-03-18 12:30:07 15阅读次数

Waters Empower因其合规性及强大的使用功能性，在市场上，受到了众多制药行业实验室工作人员的好评。Waters ACQUITY QDa作为一款智能化小型化单四极质谱，也越来越多的被应用到药物研发、QC和各个领域的质量检测和控制部门。为了使大家能更加驾轻就熟地使用Empower来处理查看QDa质谱数据，我们特别推出系列使用小贴士，助力您提高工作效率，成为Empower/质谱小达人！

任何没有MS操作经验的色谱工作者都能使用ACQUITY QDa获得有用的质谱信息。

Caroline West

法国奥尔良大学副教授

第一期：使用QDa采集和处理SIR(2D)和MS all Scan(3D)数据

第1步：

设置仪器采集方法。如下图显示采集了2D数据和3D数据。SIR（单通道离子采集），通道是单质量数的，类似于使用PDA检测器收集单波长，可视为2D数据。扫描在全质量范围内采集数据，类似于使用PDA检测器在全波长范围内采集数据，可视为3D数据。

第2步：

可以在Review界面打开SIR通道查看峰信息，进行积分。可看到基峰信息，如质荷比(m/z)、保留时间、峰面积等。也可以创建一个处理方法，包括定量分析、系统适用性等（就像对2D 色谱数据进行处理的方法）。

第3步：

可以在Review界面打开MS Scan数据（TIC总离子流图），通过下拉菜单选择需要的m/z，查看提取离子流图。

第4步：

TIC图表示采集的质量范围内每个数据点的所有离子强度的总和。进行积分后，就可以看到每个峰的峰面积。我们还可以在“光谱查看”窗口中看到每个峰顶点的质谱信息。可以创建一种处理方法，类似于为3D PDA 数据创建处理方法。此界面上有一些常用质谱数据处理的工具栏，鼠标放置上面即可查看其功能。

第5步：

在上方工具栏的右侧，有一个“3D plot”工具，可查看3D数据。或者，也可以从“窗口”(Window)菜单访问“3D plot”。

第6步：

点击“3D plot”窗口打开，可直接查看其3D数据。x轴为保留时间、y轴为强度、 z轴为m/z。这类似于3D PDA数据的3D绘图。

质谱以旧换新正在进行时

沃特世今年仍会推出多款新型质谱产品。针对使用Waters ACQUITY QDa的老用户，也计划执行老客户回馈政策，在购买新机器时给予一定的价格优惠，让您使用更低的成本，购买最新的机器！

扫描下方二维码申请获取MS使用指南并填写您的需求，后续会有专业人员与您联系！

△扫码申请

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[8]未定义数组索引: page
[8]未定义数组下标: 0
[2]Declaration of app\common\model\News::getAllList($filter = Array, $limit = 16, $order = Array, $with = Array) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\Company::getAllList($filter = Array, $limit = 20) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8]未定义数组索引: center
[8]Only variables should be passed by reference
[2]Declaration of app\common\model\NewsComment::getAllList($filter = Array, $limit = 16) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8192]Function create_function() is deprecated
[8192]Function create_function() is deprecated
[8]未定义数组索引: area
[8]未定义数组索引: st
[2]Declaration of app\common\model\Topic::getList($filter = Array) should be compatible with app\common\model\BaseModel::getList($filter = Array, $sort = Array)
[2]Declaration of app\common\model\Topic::getAllList($filter = Array, $field = 'id,md5,tit...', $limit = 0) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\TopicInfoList::getAllList($filter = Array, $limit = 8) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8]未定义数组索引: center
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: goods_list
[8]未定义数组下标: 0
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: wtt_list

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[ SQL ] SHOW COLUMNS FROM `news_category` [ RunTime:0.001019s ]
[ SQL ] SELECT `id`,`name` FROM `news_category` WHERE `is_show` = 1 [ RunTime:0.000240s ]
[ SQL ] SELECT * FROM `news_category` WHERE `parent_id` = 0 AND `is_show` = 1 ORDER BY `sort` ASC,`id` DESC [ RunTime:0.000237s ]
[ SQL ] SHOW COLUMNS FROM `company` [ RunTime:0.000918s ]
[ SQL ] SHOW COLUMNS FROM `news` [ RunTime:0.000928s ]
[ SQL ] SELECT * FROM `news` WHERE `md5` = '99e69305d000d4b5' AND `review_status` = 1 LIMIT 1 [ RunTime:0.000273s ]
[ SQL ] SHOW COLUMNS FROM `user_info` [ RunTime:0.000911s ]
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[ SQL ] SELECT * FROM `company` WHERE `id` = 415 [ RunTime:0.000210s ]
[ SQL ] SHOW COLUMNS FROM `news_text` [ RunTime:0.000964s ]
[ SQL ] SELECT * FROM `news_text` WHERE `news_id` = 6024 [ RunTime:0.030582s ]
[ SQL ] SHOW COLUMNS FROM `news_belong_category` [ RunTime:0.000976s ]
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[ SQL ] SELECT * FROM `news_category` WHERE `id` IN (5) [ RunTime:0.000202s ]
[ SQL ] SHOW COLUMNS FROM `user_follow` [ RunTime:0.000854s ]
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[ SQL ] SHOW COLUMNS FROM `news_comment` [ RunTime:0.000927s ]
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[ SQL ] SHOW COLUMNS FROM `topic` [ RunTime:0.000889s ]
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[ SQL ] SELECT * FROM `news` WHERE `id` IN (864,849) [ RunTime:0.000231s ]
[ SQL ] SELECT `id`,`md5`,`title`,`created_time` FROM `news` WHERE `review_status` = 1 AND `is_show` = 1 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.000217s ]
[ SQL ] SHOW COLUMNS FROM `user` [ RunTime:0.000790s ]
[ SQL ] SELECT `a`.* FROM `user` `a` INNER JOIN `news` `b` ON `a`.`id`=`b`.`user_id` GROUP BY `a`.`id` ORDER BY `b`.`id` DESC LIMIT 10 [ RunTime:0.010967s ]
[ SQL ] SELECT * FROM `user_info` WHERE `user_id` IN (466,458,209,372,521,819,385,657,412,274) [ RunTime:0.000887s ]
[ SQL ] SHOW COLUMNS FROM `seo` [ RunTime:0.001069s ]
[ SQL ] SELECT * FROM `seo` WHERE `column_type` = '02' AND `page_type` = 'news' AND `info_id` = 6024 LIMIT 1 [ RunTime:0.000208s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6024 [ RunTime:0.001723s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6837 [ RunTime:0.001608s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6634 [ RunTime:0.001588s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6008 [ RunTime:0.001586s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 5638 [ RunTime:0.001589s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6380 [ RunTime:0.001609s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7137 [ RunTime:0.001592s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6999 [ RunTime:0.001589s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 5829 [ RunTime:0.001591s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 5610 [ RunTime:0.001598s ]
[ SQL ] SELECT * FROM `user` WHERE `id` = 297 LIMIT 1 [ RunTime:0.000182s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 466 LIMIT 1 [ RunTime:0.000210s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 466 LIMIT 1 [ RunTime:0.000163s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 458 LIMIT 1 [ RunTime:0.000160s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 458 LIMIT 1 [ RunTime:0.000158s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 209 LIMIT 1 [ RunTime:0.000157s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 209 LIMIT 1 [ RunTime:0.000150s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 372 LIMIT 1 [ RunTime:0.000152s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 372 LIMIT 1 [ RunTime:0.000149s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 521 LIMIT 1 [ RunTime:0.000163s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 521 LIMIT 1 [ RunTime:0.000153s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 819 LIMIT 1 [ RunTime:0.000156s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 819 LIMIT 1 [ RunTime:0.000152s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 385 LIMIT 1 [ RunTime:0.000158s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 385 LIMIT 1 [ RunTime:0.000151s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 657 LIMIT 1 [ RunTime:0.000154s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 657 LIMIT 1 [ RunTime:0.000154s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 412 LIMIT 1 [ RunTime:0.000158s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 412 LIMIT 1 [ RunTime:0.000156s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 274 LIMIT 1 [ RunTime:0.000155s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 274 LIMIT 1 [ RunTime:0.000153s ]

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