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Empower控制质谱使用小贴士（三）| 沃特世质谱以旧换新进行时

来源：沃特世科技（上海）有限公司(Waters) 分类：标准 2024-04-02 17:15:08 14阅读次数

每周两分钟，见证您成为Empower/质谱小达人！

在前两期中，我们分享了单四极质谱使用小贴士：(I)采集和处理SIR(2D)和MS all Scan(3D)数据(II)结合紫外谱图查看相关质谱信息。

本期，我们来看下定量检测时如何来查看信噪比，并对谱图进行平滑。

第1步：

在没有任何平滑的情况下，看到目标峰的信噪比为29:1。

第2步：

转到【Review】中的窗口。在峰表中看到峰高和信噪比，在色谱图结果表中看到峰S/N，即33659。

第3步：

转到平滑/偏移(Smoothing/Offset)处理方法的选项卡。可以应用少量的平滑，在本例中，平滑级别为9。

第4步：

重新处理数据，看到信噪比现在为42.5:1。

另外在Smoothing Type中，Savitzky-Golay是平滑平均数据点。更多详细信息，可以搜索《色谱积分方法》(Chromatographic Integration Methods)，作者是Norman Allen Dyson。该书中相关部分对这种过滤和平滑方式作出了很好的阐述。

质谱以旧换新正在进行时

今年，沃特世将推出多款新型质谱和本土化质谱产品。针对使用Waters ACQUITY QDa的老用户，我们也计划执行老客户回馈政策，在购买新机器时已于一定的价格优惠。让您使用更低的成本购买最新的机器！

针对单四极质谱产品，我们整理了MS资料礼盒，包含仪器使用原理、实用技巧、解析数据等材料，扫描下方二维码，填写您的需求，可免费获得以下资料！快来申请吧！

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[8]未定义数组索引: page
[8]未定义数组下标: 0
[2]Declaration of app\common\model\News::getAllList($filter = Array, $limit = 16, $order = Array, $with = Array) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\Company::getAllList($filter = Array, $limit = 20) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8]未定义数组索引: center
[8]Only variables should be passed by reference
[2]Declaration of app\common\model\NewsComment::getAllList($filter = Array, $limit = 16) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8192]Function create_function() is deprecated
[8192]Function create_function() is deprecated
[8]未定义数组索引: area
[8]未定义数组索引: st
[2]Declaration of app\common\model\Topic::getList($filter = Array) should be compatible with app\common\model\BaseModel::getList($filter = Array, $sort = Array)
[2]Declaration of app\common\model\Topic::getAllList($filter = Array, $field = 'id,md5,tit...', $limit = 0) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\TopicInfoList::getAllList($filter = Array, $limit = 8) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8]未定义数组索引: center
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: goods_list
[8]未定义数组下标: 0
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: center
[2]Declaration of app\common\model\Tag::getAllList($filter = Array, $with = Array, $limit = 5) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\Tag::remove($filter) should be compatible with app\common\model\BaseModel::remove($filter = Array)
[8]未定义数组索引: center
[8]未定义数组索引: wtt_list

[ DB ] CONNECT:[ UseTime:0.000249s ] mysql:host=127.0.0.1;port=3306;dbname=120med;charset=utf8
[ SQL ] SHOW COLUMNS FROM `news_category` [ RunTime:0.001004s ]
[ SQL ] SELECT `id`,`name` FROM `news_category` WHERE `is_show` = 1 [ RunTime:0.000411s ]
[ SQL ] SELECT * FROM `news_category` WHERE `parent_id` = 0 AND `is_show` = 1 ORDER BY `sort` ASC,`id` DESC [ RunTime:0.000266s ]
[ SQL ] SHOW COLUMNS FROM `company` [ RunTime:0.001021s ]
[ SQL ] SHOW COLUMNS FROM `news` [ RunTime:0.000916s ]
[ SQL ] SELECT * FROM `news` WHERE `md5` = '4e943d98c1f97217' AND `review_status` = 1 LIMIT 1 [ RunTime:0.000307s ]
[ SQL ] SHOW COLUMNS FROM `user_info` [ RunTime:0.000908s ]
[ SQL ] SELECT * FROM `user_info` WHERE `user_id` = 297 [ RunTime:0.000779s ]
[ SQL ] SELECT * FROM `company` WHERE `id` = 415 [ RunTime:0.000290s ]
[ SQL ] SHOW COLUMNS FROM `news_text` [ RunTime:0.000802s ]
[ SQL ] SELECT * FROM `news_text` WHERE `news_id` = 6634 [ RunTime:0.031602s ]
[ SQL ] SHOW COLUMNS FROM `news_belong_category` [ RunTime:0.000968s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6634 [ RunTime:0.002059s ]
[ SQL ] SELECT * FROM `news_category` WHERE `id` IN (8) [ RunTime:0.000371s ]
[ SQL ] SHOW COLUMNS FROM `user_follow` [ RunTime:0.000865s ]
[ SQL ] SELECT `id` FROM `user_follow` WHERE `user_id` IS NULL AND `follow_user_id` = 297 LIMIT 1 [ RunTime:0.000206s ]
[ SQL ] SHOW COLUMNS FROM `news_comment` [ RunTime:0.000753s ]
[ SQL ] SELECT * FROM `news_comment` WHERE `news_id` = 6634 AND `parent_id` = 0 ORDER BY `id` DESC [ RunTime:0.000234s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `news_comment` WHERE `news_id` = 6634 LIMIT 1 [ RunTime:0.000185s ]
[ SQL ] SELECT * FROM `news` WHERE `is_show` = 1 AND `review_status` = 1 AND `id` IN (6634,3346,2296,854,7734,7724,7610,7569,7507,7445) ORDER BY field(id,6634,3346,2296,854,7734,7724,7610,7569,7507,7445) [ RunTime:0.000411s ]
[ SQL ] SELECT * FROM `user_info` WHERE `user_id` IN (297,811,184,245,7,309,777,761,696) [ RunTime:0.000849s ]
[ SQL ] SHOW COLUMNS FROM `topic` [ RunTime:0.000921s ]
[ SQL ] SELECT `id`,`md5`,`title`,`created_time` FROM `topic` WHERE `review_status` = 1 ORDER BY `id` DESC LIMIT 4 [ RunTime:0.000199s ]
[ SQL ] SHOW COLUMNS FROM `topic_info_list` [ RunTime:0.000946s ]
[ SQL ] SELECT * FROM `topic_info_list` WHERE `topic_id` = 1 ORDER BY `id` DESC LIMIT 2 [ RunTime:0.000182s ]
[ SQL ] SELECT * FROM `news` WHERE `id` IN (864,849) [ RunTime:0.000358s ]
[ SQL ] SELECT `id`,`md5`,`title`,`created_time` FROM `news` WHERE `review_status` = 1 AND `is_show` = 1 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.000223s ]
[ SQL ] SHOW COLUMNS FROM `user` [ RunTime:0.000908s ]
[ SQL ] SELECT `a`.* FROM `user` `a` INNER JOIN `news` `b` ON `a`.`id`=`b`.`user_id` GROUP BY `a`.`id` ORDER BY `b`.`id` DESC LIMIT 10 [ RunTime:0.010965s ]
[ SQL ] SELECT * FROM `user_info` WHERE `user_id` IN (466,458,209,372,521,819,385,657,412,274) [ RunTime:0.000809s ]
[ SQL ] SHOW COLUMNS FROM `seo` [ RunTime:0.000879s ]
[ SQL ] SELECT * FROM `seo` WHERE `column_type` = '02' AND `page_type` = 'news' AND `info_id` = 6634 LIMIT 1 [ RunTime:0.000215s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 6634 [ RunTime:0.001853s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 3346 [ RunTime:0.001599s ]
[ SQL ] SHOW COLUMNS FROM `tag` [ RunTime:0.000903s ]
[ SQL ] SELECT `md5` FROM `tag` WHERE `is_open` = 1 AND `tag` = '一次性使用病毒采样管' LIMIT 1 [ RunTime:0.000338s ]
[ SQL ] SELECT `md5` FROM `tag` WHERE `is_open` = 1 AND `tag` = '一次性使用病毒采样管' LIMIT 1 [ RunTime:0.000229s ]
[ SQL ] SELECT `md5` FROM `tag` WHERE `is_open` = 1 AND `tag` = '病毒采样管' LIMIT 1 [ RunTime:0.000208s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 2296 [ RunTime:0.001825s ]
[ SQL ] SELECT `md5` FROM `tag` WHERE `is_open` = 1 AND `tag` = '一次性使用无菌植绒宫颈采样拭子' LIMIT 1 [ RunTime:0.000198s ]
[ SQL ] SELECT `md5` FROM `tag` WHERE `is_open` = 1 AND `tag` = '一次性使用无菌植绒宫颈采样拭子' LIMIT 1 [ RunTime:0.000228s ]
[ SQL ] SELECT `md5` FROM `tag` WHERE `is_open` = 1 AND `tag` = '一次性使用无菌植绒宫颈采样拭子' LIMIT 1 [ RunTime:0.000191s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 854 [ RunTime:0.002875s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7734 [ RunTime:0.002911s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7724 [ RunTime:0.003116s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7610 [ RunTime:0.003148s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7569 [ RunTime:0.002642s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7507 [ RunTime:0.003152s ]
[ SQL ] SELECT * FROM `news_belong_category` WHERE `news_id` = 7445 [ RunTime:0.003083s ]
[ SQL ] SELECT * FROM `user` WHERE `id` = 297 LIMIT 1 [ RunTime:0.000197s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 466 LIMIT 1 [ RunTime:0.000202s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 466 LIMIT 1 [ RunTime:0.000135s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 458 LIMIT 1 [ RunTime:0.000130s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 458 LIMIT 1 [ RunTime:0.000166s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 209 LIMIT 1 [ RunTime:0.000165s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 209 LIMIT 1 [ RunTime:0.000145s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 372 LIMIT 1 [ RunTime:0.000142s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 372 LIMIT 1 [ RunTime:0.000148s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 521 LIMIT 1 [ RunTime:0.000144s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 521 LIMIT 1 [ RunTime:0.000136s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 819 LIMIT 1 [ RunTime:0.000135s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 819 LIMIT 1 [ RunTime:0.000134s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 385 LIMIT 1 [ RunTime:0.000164s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 385 LIMIT 1 [ RunTime:0.000140s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 657 LIMIT 1 [ RunTime:0.000141s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 657 LIMIT 1 [ RunTime:0.000136s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 412 LIMIT 1 [ RunTime:0.000145s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 412 LIMIT 1 [ RunTime:0.000147s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 274 LIMIT 1 [ RunTime:0.000148s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 274 LIMIT 1 [ RunTime:0.000159s ]

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