【微课堂】气体灭菌生物指示剂性能浅析-医疗器械网

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【微课堂】气体灭菌生物指示剂性能浅析

来源：浙江泰林生物技术股份有限公司分类：维修保养 2024-04-29 17:45:11 12阅读次数

气体灭菌主要指臭氧、二氧化氯等气体进行消毒或灭菌。主要通过气体灭菌生物指示剂检测消毒器械或消毒剂对空气中细菌的杀灭和（或）清除作用，以验证其消毒效果。

2002年版《中华人民共和国消毒技术规范》空气消毒效果鉴定试验对试验菌的要求为“白色葡萄球菌 8032”，杀灭率≥99.90% 时，可判为消毒合格。

GB28232-2020《臭氧消毒器卫生要求》中指出，用于空气消毒时，试验微生物要求为“白色葡萄球菌 8032”，灭微生物指标要求杀灭率≥99.9%；用于医疗器械和用品消毒时，试验微生物要求为枯草杆菌黑色变种芽孢（萎缩芽孢杆菌）ATCC 9372等，微生物指标要求杀灭对数值≥3.00。臭氧消毒过程中，空气湿度应不低于70%。USP指出，臭氧消毒过程中，空气湿度应不低于80%。

气体灭菌生物指示剂

泰林气体灭菌生物指示剂完全按相关标准法规进行开发，适用于臭氧、二氧化氯等气体灭菌效果的监控。

性能特点

1、采用不锈钢片载体，菌体/芽孢分散，有效避免假阳性。

2、Tyvek特卫强透析材料，透气率高，防护性强，阻菌效果优于常用医用包装透析纸。

使用流程（TSB为例）

01

按法规要求或对应的指导原则，放置于规定位置，开启消毒程序。

02

待消毒周期结束后，将灭菌处理的含菌载体无菌转移至生物安全柜中。

03

将灭菌处理后的含菌载体接种至适量的无菌胰酪大豆胨液体培养基（TSB）中，置于30～37℃培养7天。培养基装量应能够完全淹没载体。
注：推荐在生物安全柜内接种，阳性对照最后接种，避免交叉污染

04

观察相应结果，TSB清澈为阴性，浑浊为阳性。阴性表示指示剂被完全杀灭，阳性表示指示剂未被完全杀灭。

05

应同时做阳性对照，阳性对照是不将生物指示剂进行消毒，直接转移载体至适量的培养基中，经过对应的条件培养，阳性对照必须呈阳性，测试结果才有效。

气体灭菌生物指示剂配套培养液

泰林自主研发的气体灭菌生物指示剂配套培养液，适用于萎缩芽孢杆菌以及白色葡萄球菌等作为指示微生物的生物指示剂，除节约了用户的时间成本，也降低了需自行配制培养液再进行接种的人员操作以及环境条件可能造成的污染风险。

指示剂符合ISO 11138、GB 18281、PDA TR51及药典（USP,EP,Chp）等质量要求，使用的芽孢可以追溯至权威菌种保藏中心检验报告包含芽孢浓度，D值等相关数据，并提供专业的选型支持服务。泰林生命科学，将持续致力于微生物检测和控制，为您提供优质的解决方案。

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[8]未定义数组索引: page
[2]Declaration of app\common\model\TechNewsCategory::getList($filter = Array) should be compatible with app\common\model\BaseModel::getList($filter = Array, $sort = Array)
[8]未定义数组下标: 0
[2]Declaration of app\common\model\TechNews::getAllList($filter = Array, $limit = 10, $order = Array, $with = Array) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\Company::getAllList($filter = Array, $limit = 20) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8]未定义数组索引: center
[8]Only variables should be passed by reference
[2]Declaration of app\common\model\TechNewsComment::getAllList($filter = Array, $limit = 16) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8192]Function create_function() is deprecated
[8192]Function create_function() is deprecated
[8]未定义数组索引: area
[8]未定义数组索引: st
[2]Declaration of app\common\model\Topic::getList($filter = Array) should be compatible with app\common\model\BaseModel::getList($filter = Array, $sort = Array)
[2]Declaration of app\common\model\Topic::getAllList($filter = Array, $field = 'id,md5,tit...', $limit = 0) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\TopicInfoList::getAllList($filter = Array, $limit = 8) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\News::getAllList($filter = Array, $limit = 16, $order = Array, $with = Array) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8]未定义数组索引: center
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: goods_list
[8]未定义数组下标: 0
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: center
[2]Declaration of app\common\model\Tag::getAllList($filter = Array, $with = Array, $limit = 5) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\Tag::remove($filter) should be compatible with app\common\model\BaseModel::remove($filter = Array)
[8]未定义数组索引: center
[8]未定义数组索引: wtt_list

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[ SQL ] SHOW COLUMNS FROM `tech_news_belong_category` [ RunTime:0.000908s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3718 [ RunTime:0.000958s ]
[ SQL ] SELECT * FROM `tech_news_category` WHERE `id` IN (3) [ RunTime:0.000198s ]
[ SQL ] SHOW COLUMNS FROM `user_follow` [ RunTime:0.000810s ]
[ SQL ] SELECT `id` FROM `user_follow` WHERE `user_id` IS NULL AND `follow_user_id` = 365 LIMIT 1 [ RunTime:0.000370s ]
[ SQL ] SHOW COLUMNS FROM `tech_news_comment` [ RunTime:0.000766s ]
[ SQL ] SELECT * FROM `tech_news_comment` WHERE `tech_news_id` = 3718 AND `parent_id` = 0 ORDER BY `id` DESC [ RunTime:0.000192s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `tech_news_comment` WHERE `tech_news_id` = 3718 LIMIT 1 [ RunTime:0.000293s ]
[ SQL ] SELECT * FROM `tech_news` WHERE `is_show` = 1 AND `review_status` = 1 AND `id` IN (3718,2606,2333,1520,3419,2629,1152,3912,3907,3880) ORDER BY field(id,3718,2606,2333,1520,3419,2629,1152,3912,3907,3880) [ RunTime:0.000511s ]
[ SQL ] SELECT * FROM `user_info` WHERE `user_id` IN (365,714,206,156,353,12,545) [ RunTime:0.001136s ]
[ SQL ] SHOW COLUMNS FROM `topic` [ RunTime:0.001114s ]
[ SQL ] SELECT `id`,`md5`,`title`,`created_time` FROM `topic` WHERE `review_status` = 1 ORDER BY `id` DESC LIMIT 4 [ RunTime:0.000206s ]
[ SQL ] SHOW COLUMNS FROM `topic_info_list` [ RunTime:0.000917s ]
[ SQL ] SELECT * FROM `topic_info_list` WHERE `topic_id` = 1 ORDER BY `id` DESC LIMIT 2 [ RunTime:0.000180s ]
[ SQL ] SHOW COLUMNS FROM `news` [ RunTime:0.000737s ]
[ SQL ] SELECT * FROM `news` WHERE `id` IN (864,849) [ RunTime:0.000192s ]
[ SQL ] SELECT `id`,`md5`,`title`,`created_time` FROM `tech_news` WHERE `review_status` = 1 AND `is_show` = 1 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.000180s ]
[ SQL ] SHOW COLUMNS FROM `user` [ RunTime:0.000675s ]
[ SQL ] SELECT `a`.* FROM `user` `a` INNER JOIN `tech_news` `b` ON `a`.`id`=`b`.`user_id` GROUP BY `a`.`id` ORDER BY `b`.`id` DESC LIMIT 10 [ RunTime:0.004741s ]
[ SQL ] SELECT * FROM `user_info` WHERE `user_id` IN (592,413,444,224,13,239,598,697,394,285) [ RunTime:0.000729s ]
[ SQL ] SHOW COLUMNS FROM `seo` [ RunTime:0.000746s ]
[ SQL ] SELECT * FROM `seo` WHERE `column_type` = '16' AND `page_type` = 'tech_news' AND `info_id` = 3718 LIMIT 1 [ RunTime:0.000178s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3718 [ RunTime:0.000988s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 2606 [ RunTime:0.000899s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 2333 [ RunTime:0.000903s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 1520 [ RunTime:0.000835s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3419 [ RunTime:0.000829s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 2629 [ RunTime:0.000839s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 1152 [ RunTime:0.000863s ]
[ SQL ] SHOW COLUMNS FROM `tag` [ RunTime:0.000784s ]
[ SQL ] SELECT `md5` FROM `tag` WHERE `is_open` = 1 AND `tag` = '超净工作台和生物安全柜的四大区别' LIMIT 1 [ RunTime:0.000199s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3912 [ RunTime:0.001040s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3907 [ RunTime:0.001041s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3880 [ RunTime:0.000881s ]
[ SQL ] SELECT * FROM `user` WHERE `id` = 365 LIMIT 1 [ RunTime:0.000159s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 592 LIMIT 1 [ RunTime:0.000161s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 592 LIMIT 1 [ RunTime:0.000128s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 413 LIMIT 1 [ RunTime:0.000123s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 413 LIMIT 1 [ RunTime:0.000128s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 444 LIMIT 1 [ RunTime:0.000191s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 444 LIMIT 1 [ RunTime:0.000121s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 224 LIMIT 1 [ RunTime:0.000114s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 224 LIMIT 1 [ RunTime:0.000116s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 13 LIMIT 1 [ RunTime:0.000114s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 13 LIMIT 1 [ RunTime:0.000219s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 239 LIMIT 1 [ RunTime:0.000162s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 239 LIMIT 1 [ RunTime:0.000140s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 598 LIMIT 1 [ RunTime:0.000143s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 598 LIMIT 1 [ RunTime:0.000146s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 697 LIMIT 1 [ RunTime:0.000230s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 697 LIMIT 1 [ RunTime:0.000159s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 394 LIMIT 1 [ RunTime:0.000140s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 394 LIMIT 1 [ RunTime:0.000122s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 285 LIMIT 1 [ RunTime:0.000154s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 285 LIMIT 1 [ RunTime:0.000150s ]

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