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应用“泌”籍 | 外泌体超离纯化指南

来源：贝克曼库尔特商贸（中国）有限公司分类：产品评测 2024-03-28 12:15:09 9阅读次数

万象更新的2024年初，国际细胞外囊泡学会（ISEV）发布了最新一版细胞外囊泡研究指南：Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches。该指南由超过1000名作者共同完成，为研究人员提供了详细的外囊泡来源、分离和表征等方面的信息。同时对外囊泡的最新研究进展、技术和方法进行了介绍。

作为外囊泡纯化的金标准和最常见方法，指南在第四章节对差速离心（4.2 Differential (ultra)centrifugation，简称dUC）和密度梯度离心（4.3 Density gradient/cushion ，简称DG）都进行了综合性的介绍。

为了让各位小伙伴对外泌体超速离心纯化有更细致的了解，小贝君精心同步制作了：外泌体超离纯化指南。

本指南全面的介绍了不同的超速离心方法学原理，并从主机，转子，耗材各个维度进行了细致的介绍，方便各位小伙伴能够快速的根据所需，选择合适的实验方法和实验设备耗材。

同时，针对不同的离心需求和规模，结合全面的离心家族产品线，小贝君也为各位小伙伴整理了相应的外泌体离心整体解决方案，方便大家构建外泌体离心纯化的全部流程。快来下载查看详细信息吧。

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[8]未定义数组索引: page
[2]Declaration of app\common\model\TechNewsCategory::getList($filter = Array) should be compatible with app\common\model\BaseModel::getList($filter = Array, $sort = Array)
[8]未定义数组下标: 0
[2]Declaration of app\common\model\TechNews::getAllList($filter = Array, $limit = 10, $order = Array, $with = Array) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\Company::getAllList($filter = Array, $limit = 20) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8]未定义数组索引: center
[8]Only variables should be passed by reference
[2]Declaration of app\common\model\TechNewsComment::getAllList($filter = Array, $limit = 16) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8192]Function create_function() is deprecated
[8192]Function create_function() is deprecated
[8]未定义数组索引: area
[8]未定义数组索引: st
[2]Declaration of app\common\model\Topic::getList($filter = Array) should be compatible with app\common\model\BaseModel::getList($filter = Array, $sort = Array)
[2]Declaration of app\common\model\Topic::getAllList($filter = Array, $field = 'id,md5,tit...', $limit = 0) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\TopicInfoList::getAllList($filter = Array, $limit = 8) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[2]Declaration of app\common\model\News::getAllList($filter = Array, $limit = 16, $order = Array, $with = Array) should be compatible with app\common\model\BaseModel::getAllList($filter = Array, $with = Array, $limit = 0, $sort = Array, $field = 'a.*', $join = Array)
[8]未定义数组索引: center
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: cid
[8]未定义数组索引: goods_list
[8]未定义数组下标: 0
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: center
[8]未定义数组索引: wtt_list

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[ SQL ] SHOW COLUMNS FROM `tech_news_category` [ RunTime:0.000941s ]
[ SQL ] SELECT `id`,`name` FROM `tech_news_category` WHERE `is_show` = 1 [ RunTime:0.000706s ]
[ SQL ] SELECT * FROM `tech_news_category` WHERE `parent_id` = 0 AND `is_show` = 1 ORDER BY `sort` ASC,`id` DESC [ RunTime:0.000214s ]
[ SQL ] SHOW COLUMNS FROM `company` [ RunTime:0.000913s ]
[ SQL ] SHOW COLUMNS FROM `tech_news` [ RunTime:0.000773s ]
[ SQL ] SELECT * FROM `tech_news` WHERE `md5` = 'ed97ad42a0a42f7d' AND `review_status` = 1 LIMIT 1 [ RunTime:0.000298s ]
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[ SQL ] SELECT * FROM `company` WHERE `id` = 422 [ RunTime:0.000204s ]
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[ SQL ] SELECT * FROM `tech_news_text` WHERE `tech_news_id` = 3111 [ RunTime:0.014005s ]
[ SQL ] SHOW COLUMNS FROM `tech_news_belong_category` [ RunTime:0.001123s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3111 [ RunTime:0.001113s ]
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[ SQL ] SHOW COLUMNS FROM `tech_news_comment` [ RunTime:0.000847s ]
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[ SQL ] SHOW COLUMNS FROM `topic` [ RunTime:0.000984s ]
[ SQL ] SELECT `id`,`md5`,`title`,`created_time` FROM `topic` WHERE `review_status` = 1 ORDER BY `id` DESC LIMIT 4 [ RunTime:0.000275s ]
[ SQL ] SHOW COLUMNS FROM `topic_info_list` [ RunTime:0.001025s ]
[ SQL ] SELECT * FROM `topic_info_list` WHERE `topic_id` = 1 ORDER BY `id` DESC LIMIT 2 [ RunTime:0.000158s ]
[ SQL ] SHOW COLUMNS FROM `news` [ RunTime:0.000823s ]
[ SQL ] SELECT * FROM `news` WHERE `id` IN (864,849) [ RunTime:0.000202s ]
[ SQL ] SELECT `id`,`md5`,`title`,`created_time` FROM `tech_news` WHERE `review_status` = 1 AND `is_show` = 1 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.000355s ]
[ SQL ] SHOW COLUMNS FROM `user` [ RunTime:0.000716s ]
[ SQL ] SELECT `a`.* FROM `user` `a` INNER JOIN `tech_news` `b` ON `a`.`id`=`b`.`user_id` GROUP BY `a`.`id` ORDER BY `b`.`id` DESC LIMIT 10 [ RunTime:0.004302s ]
[ SQL ] SELECT * FROM `user_info` WHERE `user_id` IN (444,224,13,239,598,697,394,285,190,502) [ RunTime:0.000706s ]
[ SQL ] SHOW COLUMNS FROM `seo` [ RunTime:0.000803s ]
[ SQL ] SELECT * FROM `seo` WHERE `column_type` = '16' AND `page_type` = 'tech_news' AND `info_id` = 3111 LIMIT 1 [ RunTime:0.000173s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3111 [ RunTime:0.000995s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3695 [ RunTime:0.000858s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3684 [ RunTime:0.001061s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3683 [ RunTime:0.000860s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3674 [ RunTime:0.000816s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3634 [ RunTime:0.000841s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3632 [ RunTime:0.000856s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3573 [ RunTime:0.000814s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3564 [ RunTime:0.000842s ]
[ SQL ] SELECT * FROM `tech_news_belong_category` WHERE `tech_news_id` = 3550 [ RunTime:0.000821s ]
[ SQL ] SELECT * FROM `user` WHERE `id` = 304 LIMIT 1 [ RunTime:0.000172s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 444 LIMIT 1 [ RunTime:0.000179s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 444 LIMIT 1 [ RunTime:0.000174s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 224 LIMIT 1 [ RunTime:0.000129s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 224 LIMIT 1 [ RunTime:0.000122s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 13 LIMIT 1 [ RunTime:0.000162s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 13 LIMIT 1 [ RunTime:0.000132s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 239 LIMIT 1 [ RunTime:0.000112s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 239 LIMIT 1 [ RunTime:0.000160s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 598 LIMIT 1 [ RunTime:0.000153s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 598 LIMIT 1 [ RunTime:0.000123s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 697 LIMIT 1 [ RunTime:0.000149s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 697 LIMIT 1 [ RunTime:0.000155s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 394 LIMIT 1 [ RunTime:0.000120s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 394 LIMIT 1 [ RunTime:0.000186s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 285 LIMIT 1 [ RunTime:0.000162s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 285 LIMIT 1 [ RunTime:0.000382s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 190 LIMIT 1 [ RunTime:0.000146s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 190 LIMIT 1 [ RunTime:0.000368s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `follow_user_id` = 502 LIMIT 1 [ RunTime:0.000142s ]
[ SQL ] SELECT COUNT(*) AS tp_count FROM `user_follow` WHERE `user_id` = 502 LIMIT 1 [ RunTime:0.000141s ]

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